Aim 2

To investigate the 80S•tRNA2•mRNA•eEF2•GDP complex that is observed upon addition of eEF2•GTP to a programmed PRE complex.

To understand why eEF2 can stably bind to ribosomes and two tRNAs in vitro (Budkevich et al., 2014), and how this fits to the model of translocation, I look at an in vitro reconstituted rabbit 80S•tRNA2•mRNA•eEF2•GDP complex.