Aim 3

To revisit the mammalian polysome landscape to find out if serum deprivation influences the distribution of states.

Cryo-EM of actively translating polysomes gives insight into the energy landscape of mammalian translation (Behrmann et al., 2015). Among other stimuli, serum deprivation and subsequent serum restimulation has been hypothesized to influence translation, e.g. via changing polysome patterns (Duncan and McConkey, 1982; Viero et al., 2015). I want to investigate if overnight serum deprivation and 30 minutes of serum restimulation can change the energy landscape of translation.